Figure 6. TMF attenuates neuronal apoptosis. Brain tissues were stained with H&E to determine neuronal morphology in the hippocampus region (A). TUNEL assay was used to investigate cell apoptosis in brain tissues (B), TUNEL-positive cells were analyzed as % of control to compare all groups (C). Representative cleaved caspase 3 levels as determined by Western blotting are presented in a histogram (with actin as the control) (D). The data are the mean ± SEM from three independent experiments (***p<0.001 compared with the control group, ^#p<0.05 compared with the DEX group).