Table 1. Polymerase chain reaction primers used to identify AmpC β-lactamase production.
Name | Sequence (5′−3′) | Length | Target gene | Variants coverage |
---|---|---|---|---|
ACT-F | AAC CRT CCG RCA TRA GCR AR | 120 bp | blaACT | 1, 2, 3, 4, 6, 10, 13, 38 |
ACT-R | RGT ARC CCC AG CRT AAR GR | |||
DHA-F | CTR CAA CAC TRA TRT CCG CT | 217 bp | blaDHA | DHA 1–7, 14,15,17,22. |
DHA-R | ARTR GTA RTT RCA RTG ACC RGC R | |||
CIT-F | RAT RCC ARC CAC RTT CARGA | 262 bp | blaCIT | LAT 1, 3, 4 and CMY 2, 4, 5, 6, 7 |
CIT-R | RGT ARC TRC CAA ARC CAC CA | |||
FOX-F | RAC CCT RTT CRA GAR TRG CT | 325 bp | blaFOX | 1–10 (except 2 & 9) |
FOX-R | ACR ART TRC RGC CAR GTRG AC | |||
MIR-F | AGC CAC ACT ACT RTA CRCR TCR | 401 bp | blaMIR | 1–17 (except 14) |
MIR-R | ARAGCTCARRCCGRAARGRT | |||
MOX-F | CAA CRA CAA RCC ARC CRG TR | 173 bp | blaMOX | MOX 1 to 8, CMY 1, 8 to 11 |
MOX-R | CAR CCT TRA RCA CGR CCA C |
R, G or T; S, A or T.