| pMODfor |
CCAACGACTACGCACTAGCCAAC |
Sequencing of the rescue plasmids |
| pTn5Oksfor |
ATTCAGGCTGCGCAACTG |
| rt_hrdB_ghana_up |
GAGTTCGGCGACCTCATTG |
RT-PCR analysis of
S. ghanaensis
genes |
| rt_hrdB_ghana_rp |
CGTCTTGGACTCGATCTGG (240 bp) |
|
| rt_moeO5_up |
GAATACCCCGCGAGGAGTG |
|
| rt_moeO5_rp |
GCTGTCGAGGTACTCGGTG (302 bp) |
|
| rt_01967_up |
TCCAGGAGGAGATCTCCTG |
|
| rt_01967_rp |
GCTTCTCCCAGGCGTCAC (280 bp) |
|
| rt_adpA_gh_up |
GGAATCGATCTGTGCCTGC |
|
| rt_adpA_gh_rp |
GGTGATCAGCCACTGCAGC |
|
| Ssfg_01967_XbaI_up |
AAATCTAGAGGAAATGATCGAGAACTCC |
Cloning of miaB
|
| Ssfg_01967_EcoRI_rp |
AAAGAATTCGTCCGACTCCGTCACGAC |
|
| ssfg_01967_HindIIIup |
AAAAAGCTTGAGGGTGACGGTGACGGTAC |
Cloning of 5 kb fragment for miaB knockout |
| ssfg_01967_XbaIrp |
AAATCTAGAGGTCGAAGGCCACGGAGG |
|
| Ssfg_01967red_up |
GCGGAGGCCGTCCGCGGCACCGCCTACCCTTA |
RedET-assisted replacement of miaB with hyg cassette |
|
GGGCATGTTCCGGGGATCCGTCGACCC |
|
| Ssfg_01967red_rp |
GCCGAGCCGTGCGTCCGCCCCCTCGTCGCGGAC |
|
|
CGGTCA TGTAGGCTGGAGCTGCTTCG |
|
| aqua_adpA_f |
TACCTCGACCGGTCTCTCCCGGAG |
TTA→CTC replacement |
| aqua_adpA_r |
GCCGATCTCCTCCGGGAGAGACCG |
|
