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Human primer sequences used for quantitative real time PCR
| Gene | Strand | Primer sequence |
|---|---|---|
| β‐Actin | Forward | CAAACATGATCTGGGTCATCTTCT |
| Reverse | CAAACATGATCTGGGTCATCTTCT | |
| DHCR7 | Forward | ACATGCTCGGCTCTCGGAC |
| Reverse | AGGTATAGAGCTGGGCGGCT | |
| DHCR24 | Forward | ATCGCAGCTTTGTGCGATG |
| Reverse | CACCAGGAAACCCAGCGT | |
| HPRT | Forward | GAAAGGGTGTTTATTCCTCATGG |
| Reverse | CAGTGCTTTGATGTAATCCAGCAG | |
| LSS | Forward | GGCAGACGTGGACCTACC |
| Reverse | GAAAAGTGGGCCACCATAATC | |
| SREBF2 | Forward | CCCTTCAGTGCAACGGTCATTCAC |
| Reverse | TGCCATTGGCCGTTTGTGTC |
