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. 2020 Feb 3;8:e8514. doi: 10.7717/peerj.8514

Figure 4. Glycyrrhizin suppressed the TGF-β1-induced EMT process by inhibiting HMGB1.

Figure 4

Pretreated A549 cells (50 µM, 100 µM, 200 µM) and BEAS-2B cells (25 µM, 50 µM, 100 µM) with glycyrrhizin for 2 h, then stimulated the cells with 5 ng/ml TGF-β1 for 24 h. (A, B) Elisa was performed to detect the HMGB1 concentration in cell supernatant. (C, D) Western blot was used to detect the expression of E-cadherin, Vimentin, and HMGB1. (E, F) Statistical analysis of the expression of E-cadherin, Vimentin and HMGB1 compared to GAPDH. #P < 0.05, ##P < 0.01 compared with the value of control group, *P < 0.05, **P < 0.01, ***P < 0.001 compared with the value of TGF-β1 group. (G-R) A549 and BEAS-2B cells were stained with DAPI (blue, nuclear stain) and antibodies to E-cadherin or Vimentin (red), and confocal images were acquired at 40× magnification. All experiments were performed in three independent experiments.