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. 2020 Feb 3;8:e8514. doi: 10.7717/peerj.8514

Figure 6. Glycyrrhizin blocked the TGF-β1/Smads pathway by inhibiting HMGB1.

Figure 6

(A, B) Western blot was used to detect the protein level of TGF-β1, Smad2, p-Smad2, Smad3, p-Smad3 of cells in the control, Lv-NC, Lv-HMGB1, Lv-HMGB1+glycyrrhizin groups. (C, D) Western blot analysis was used to detect the protein expression of Smad2, p-Smad2, Smad3, p-Smad3 of cells stimulated by 5 ng/ml TGF-β1 with or without various concentrations of glycyrrhizin pretreatment. (E, F) Statistical analysis of the relative expression of TGF-β1/GAPDH, p-Smad2/Smad2, and p-Smad3/Smad3. ##P < 0.01, ###P < 0.001 compared with the value of Lv-NC group, *P < 0.05, **P < 0.01, compared with the value of Lv-HMGB1 group. (G, H) Analyze the relative expression of p-Smad2/Smad2, p-Smad3/Smad3 according to the data of three independent repeated experiments. ##P < 0.01, ###P < 0.001 compared with the value of control group, *P < 0.05, **P < 0.01, ***P < 0.001 compared with the value of TGF-β1 group.