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. 2019 Dec 16;87(1):174–190. doi: 10.1002/mrd.23306

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© 2019 The Authors. Molecular Reproduction and Development Published by Wiley Periodicals, Inc.

This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes.

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To identify metabolites present in the uterine milieu during the early stages of blastocyst elongation, flushings were taken from pregnant gilts on Days 9–11 of gestation. Flushings were sorted into one of five blastocyst treatment groups (flushings populations) according to conceptus morphology (embryo stage): uniform spherical (S, n = 8 gilts), heterogeneous spherical and ovoid (SO, n = 8 gilts), uniform ovoid (O, n = 8 gilts), heterogeneous ovoid and tubular (OT, n = 8 gilts), or uniform tubular (T, n = 6 gilts). Subsequently, uterine flushings populations were categorized into three classifications of elongation stage (classification stages): (a) pre‐elongation stage (S only), (b) initiation stages (SO and O), and (c) transitional stages (OT and T). The average homogeneities for each blastocyst treatment group were: S = 100%; SO = 62%; O = 100%; OT = 56%; and T = 90% [Color figure can be viewed at wileyonlinelibrary.com]