Figure 4.

Target validation using immunoblotting and fluorescence. a) Immunoblot of AfBPP samples following treatment of human DMD myoblast cells and biotin enrichment. Selective enrichment of AhR by active probe 3, but not inactive probe 4 is observed as well as enrichment competition by ezutromid and SMT022332 (7). M, protein standard (in kDa); uncropped blot is presented in Supplementary Figure 6. b) Fluorescence of ezutromid (100 nm, 313 nm excitation, 390 nm emission) measured after incubation (16 h) with AhR:ARNT (5–10000 nm).