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. 2019 Aug 28;225(4):1799–1815. doi: 10.1111/nph.16087

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© 2019 The Authors. New Phytologist © 2019 New Phytologist Trust

This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc-nd/4.0/ License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made.

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The abscission zones (AZ) of rice, Brachypodium and Setaria differ in histology and cell wall structures. (a–c) Fast green/Safranin O staining of mature spikelets in (a) rice, (b) Brachypodium and (c) Setaria. Safranin O stains secondary cell wall including lignin, suberin and cutin with a magenta colour, and fast green stains primary cell wall and cytoplasm with a green colour. The AZ is marked with black dotted lines. (d–f) Transmission electron microscopy images of the AZ and surrounding cells in (d) rice, (e) Brachypodium and (f) Setaria. (g–i) Zoomed in images of the blue dotted rectangles shown in (d‐f). Bars: (a ‐c) 100 µm; (d #x2010;f) 2 µm; (g #x2010;i) 1 µm.