Figure 3.
Reprogramming of bone marrow‐derived dendritic cells (bmDCs) to pluripotency. A, Phase‐contrast photomicrograph of a typical colony of induced pluripotent stem cells (iPSCs) reprogrammed from bmDCs (iPSCDC) at passage 7, cultured on mouse embryonic fibroblast (MEF) feeder cells. B, Phase‐contrast photomicrograph of a typical colony of iPSCs reprogrammed from MEFs (iPSCMEF), cultured on feeder cells, likewise at passage 7. C‐F, Methylene blue staining of six well plates revealing the abundance of iPSC colonies from a representative experiment in which equivalent numbers of MEFs (C) and bmDCs (D and F) were reprogrammed in parallel. E, Control well of MEFs without the addition of reprogramming vectors. F, Well containing equivalent numbers of bmDCs induced to mature in response to lipopolysaccharide (LPS) prior to reprogramming. Numbers in the bottom left hand corner of each image denote the number of iPSC colonies observed