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. 2019 Aug 13;71(1):247–258. doi: 10.1002/hep.30792

Figure 2.

Figure 2

Increased biliary cholesterol secretion after NTCP inhibition is largely independent of ABCG5/8. (A) Hepatic mRNA and protein expression of Abcg5, Abcg8, and Abcb4 in WT mice. RNA expression is compared to the geometric mean of reference genes 36b4 and Tbp. Protein levels were compared to the sodium/potassium ATPase (n = 3/group). (B) Cholesterol output (in nmol/min/100 g BW) in Abcg8 −/− mice. One 10‐minute interval of biliary cholesterol output is compared between WT and Abcg8 −/− mice. (C) Phospholipid and (D) bile salt output into bile were determined in Abcg8 −/− mice. (E) Cholesterol output and (F) phospholipid output were plotted as a function of biliary bile salt output (linear regression was performed on log‐transformed data and significance was assessed by comparing slopes or intercepts). Data are presented as median and interquartile range. White squares/bars indicate the vehicle group, and black/gray squares/bars indicate the Myrcludex B group. Differences between groups were analyzed using the Mann‐Whitney U test. Asterisk (“*”) indicates P < 0.05; n = 8 mice/group. × Indicates statistical outlier in Tukey plot. Abbreviations: Chol., cholesterol; PL, phospholipid; Tbp, TATA‐box binding protein.