Figure 4.

CreERT2 activation can be temporally controlled by brief exposure to 4OH‐tamoxifen in whole embryo culture. (a) Schematic of the protocol used and representative embryo at the end of culture showing an inflated yolk sac and well‐formed yolk sac vasculature. (b) Quantification of PNP recombination in Nkx1.2 ^CreERT2‐expressing mTmG embryos treated for 1 h with either 2 or 10 μM 4OH‐tamoxifen. (c) Representative images of embryos quantified in (b). Scale bar = 100 μm, *** p < .001, each point represents an individual embryo from three independent cultures at each concentration