Figure 5.

Spatially restricted gene recombination achieved by injecting TAT‐Cre. (a) Schematic of the experimental protocol. Arrows indicate the locations of TAT‐Cre injection by mouth pipetting (b) transversally into a cranial neural fold or (c) in a rostrocaudal line along a posterior neural fold 24 h prior to collection of the embryos. (b) Stereoscope image of a Cre‐negative Rosa26 ^mTmG embryo image showing regional recombination in the hindbrain 24 h following local TAT‐Cre injection. (c) Confocal image of a PNP showing regional recombination 24 h following TAT‐Cre injection into one neural fold. Phall, phalloidin; scale bar = 100 μm