Figure 6.

Part engineering to optimise dynamic range: a) Anderson collection of promoters (http://parts.igem.org/Promoters/Catalog/Anderson) are well characterised constitutive promoter sequences of a range of strengths which could be used to replace the −35 and −10 sites of responsive promoters to alter their behaviour. Different strength promoter sequences will alter the maximum expression and the leakiness. Response curves are shown for activator and repressor systems.59 b) Alternative ribosome binding site (RBS) sequences of different strengths can be replaced to alter the response of the biosensors.60, 61 The response curves for activator and repressor systems with different RBS sequences are shown.