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. 2020 Jan 29;14:7. doi: 10.3389/fncel.2020.00007

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Copyright © 2020 Pereiro, Ruzafa, Acera, Urcola and Vecino.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Analysis of pig Müller cell cultures derived from retinas digested with papain or trypsin. Images from Müller cell cultures derived from retinas digested with trypsin (A) or papain (B). Müller cells are labeled with an antibody against vimentin (green) and the nuclei are stained with DAPI (blue). The histogram represents the analysis of the number of Müller cells after 7 DIV (C). The survival of Müller cells after 7 DIV increased significantly when the retina is digested with papain: ^∗p-value < 0.05. Scale bar, 100 μm.