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. 2020 Jan 29;14:7. doi: 10.3389/fncel.2020.00007

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Copyright © 2020 Pereiro, Ruzafa, Acera, Urcola and Vecino.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Analysis of pig Müller cells when cultured in different media: DMEM + 10% FBS, DMEM-F12, and NBA/B27 + 10% FBS. The purity and survival of the cells maintained in DMEM + 10% FBS (A), DMEM/F12 (B) or NBA B27 + 10% FBS (C) was analyzed. Neurons (RGCs) were labeled with an antibody against βIII-tubulin (red), Müller cells with an antibody against vimentin (green) and the nuclei were stained with DAPI. Note that the cultures maintained in NBA/B27 + 10% FBS were not pure (C). In DMEM + 10% FBS, Müller cells reached confluency more rapidly (A) and there were fewer Müller cells in the cultures grown in NBA + 10% FBS and DMEM-F12 at both time points, as seen in the histogram (D): ^∗p-value < 0.05. Scale bar, 50 μm.