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Enzymatic methylation of cap‐4 RNA using Ecm1 methyltransferase. A) RNA sequences and reaction scheme. B) Anion‐exchange HPLC analysis of a typical methylation reaction (start and after 45 min; see the Experimental Section for reaction conditions), inset shows methylated product after purification. C) LC–ESI mass spectrum of Gppp‐RNA 10 and purified m7GpppRNA 11.
