Skip to main content
. Author manuscript; available in PMC: 2021 Jan 16.
Published in final edited form as: Cell Chem Biol. 2019 Dec 9;27(1):47–56.e15. doi: 10.1016/j.chembiol.2019.11.006

Figure 5. UNC6852 degrades PRC2 and reduces H3K27me3 levels in EZH2Y641N DB cells.

Figure 5.

(A) Western blot analysis of the degradation of EED, EZH2, and SUZ12 in DB cells containing a heterozygous EZH2Y641N mutation treated with UNC6852 (0.1 – 30 μM for 24 hours). (B) Western blot analysis following treatment of DB cells with UNC6852 or negative control compound UNC7043 (10 μM for 24 hours). (C) Western blot analysis of PRC2 components and H3K27me3 in DB cells treated with UNC6852 in a time dependent fashion (10 μM for 24, 48 and 72 hours). (D) Quantification of H3K27me3 levels relative to total H3 in C. DMSO control was normalized to 1. Data are representative of at least two biological replicates. Data are represented as mean ± standard deviation