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. 2020 Feb 6;15(2):e0228463. doi: 10.1371/journal.pone.0228463

Fig 7. Antibody measurements at 2 weeks after primary intranasal immunization.

Fig 7

(A) Specific IgA antibodies in sera, nasal wash, fecal extract, and genital secretions. Significant production of IgA was detected in the fecal excretions and genital secretions from the CNs-Mdh-immunized group compared to CNs-TF-immunized group. (B) Specific IgG antibodies at 2 wpi. The CNs-Mdh elicited a significantly higher titer of specific IgG than that of the CNs-TF-immunized group which served as the vector control. The main subtype produced after immunization was IgG1, while the titer of IgG2a was not significantly enhanced following immunization. (C) Total IgA antibodies in sera, nasal wash, fecal extract, and genital secretions. Titers of IgA in genital secretions and fecal excretions were significantly increased in CNs-Mdh-immunized group compared with those following the CNs-TF-immunization. In the nasal washes, a high titer of IgA in the CNs-Mdh-immunized group was measured, indicating significance compared to the PBS- and CNs-immunized groups. However, there were no significant differences in sera. Groups were statistically compared using one-way ANOVA with Tukey’s post hoc multiple comparison test. The results for specific antibodies are expressed as the sample to negative control (PBS) ratio (S/N ratio).