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. 2020 Feb 6;15(2):e0228381. doi: 10.1371/journal.pone.0228381

Fig 6.

Fig 6

IFN-ɣ:IL-5, IFN-ɣ:IL-10 and IFN-ɣ:IL-17A ratios in the supernatants of spleen cells cultures in response to mixtures of peptides (PPs) from mice immunized with recombinant proteins, (a) emulsified with IFA, (b) adsorbed onto Alum, and (c) rM. smegmatis, (d) rM. vaccae, and (e) rDNA vaccine constructs. The ratios of IFN-ɣ:IL-5, IFN-ɣ:IL-10, and IFN-ɣ:IL-17A exceeding 2 (straight line) were considered as Th1 bias, 1 to 2 no bias, and <1 (dotted line) as Th2, Treg and Th17 biases, respectively.