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Confocal laser scanning microscopy of Calu-3 monolayers immunolabelled for ATB0,+ transporter (green) is shown. Nuclei were stained with propidium iodide (red). Cells were cultured under ALI condition for 21d (left) or 8d (right). Top: XZ scans are shown. Bottom: 3D reconstruction of z-stacks confocal images; about 25 horizontal sections were acquired. Representative images from three independent experiments are shown. Bar, 10 μm.
