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. 2020 Feb 6;15(2):e0228436. doi: 10.1371/journal.pone.0228436

Fig 1. Model and design for studies of effects of Suff and GVAD diets on timed mating of WT and Cyp1b1-/- mice.

Fig 1

Design to compare effects of GVAD and Cyp1b1 deletion at different stages of development (Left) Mid-gestation; E9.5 liver budding Foregut/FG, Endoderm (Endo), Septum Transversum Mesenchyme (STM). (Center) PN0.5; Stellate activation markers examined. (Right) PN21; suppression of oleate and cholesterol pathway genes (A). Design for assessment of effects of a retinol-depleted maternal diet (GVAD versus Suff) and of Cyp1b1 deletion (WT versus Cyp1b1-/- pups) on livers at birth (PN0.5) and weaning (PN21). Gene expression and retinoid content in pooled livers are compared on the day after birth (n = 3 pools) and on individual pups at PN21 (B).