Fig 2. Effects of GVAD and Cyp1b1 deletion on liver retinoid content and gene expression at birth and weaning.

PCR determinations of expression (dCt) for individual WT and Cyp1b1-/- mice from multiple litters at PN21: Aldh1a1, Rdh11, Scd1, and Hmgcr (A). Correlations for expression versus body weight for WT and Cyp1b1-/- mice (B). Liver retinol and retinyl ester content at birth in WT (open circles) and Cyp1b1-/- (solid circles) vitamin A-sufficient (Suff; black) and GVAD (red) mice were measured on the pooled livers from separate litters (C). Equivalent measurements on livers from individual WT and Cyp1b1-/- at weaning/PN21 pups (D). Correlation of GVAD and Cyp1b1-/- effects on stellate markers at birth (C). Correlation between Col1a1 expression and Sma1 and then other stellate activation markers at birth for expression in individual mice across the four treatments shown in Table 2 (E). Parts C and D were previously presented as separate supplementary figures [5]. *p-value ≤ 0.05, **p-value ≤ 0.01, ***p-value ≤ 0.001.