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. 2020 Feb 6;15(2):e0228436. doi: 10.1371/journal.pone.0228436

Fig 3. Retinoid homeostasis in WT, Cyp1b1-/- and DKO mice.

Fig 3

Experimental design for embryos at E9.5 and pups at birth and weaning. C57Bl/6J (WT), Cyp1b1-/-, and DKO pregnant dams were administered vitamin A-sufficient (Suff) or -deficient (GVAD) diets at E4.5 (or E0.5 for DKO dams). Whole mount in situ hybridization (ISH) at E9.5 was used to observe the intensity and location of genes involved in retinoid homeostasis and developmental patterning (A). Depletion of serum retinol and retinyl esters at E14.5 in DKO dams and fetuses on Suff and GVAD maternal diets. Data previously published as a Table [33] (B). *p-value ≤ 0.05 compared to sufficient within the same genotype. $p-value ≤ 0.05 compared to WT on the same diet. N.D., Not detectable (<0.01 μg/dl). E9.5 embryo sizes obtained from dorsal projections for the six treatment groups (C).