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. 2020 Feb 6;15(2):e0228252. doi: 10.1371/journal.pone.0228252

Fig 7. Analysis of systemic humoral and T-memory cell responses.

Fig 7

(A) Plaque reduction neutralization assay at 1:40 dilution. (B) Indirect ELISA assay for HSV-1-specific IgG response at 1:100 dilution. Flow cytometric analysis of draining lymph nodes was performed on Day 3 post challenge to quantify the memory cell response. CD4-Tcm (C) CD4-Tem (D), CD8-Tcm (E) CD8-Tem (F) memory cell subtypes were shown. Statistical analysis was done using Two-Way ANOVA (A, B) and One-Way ANOVA and Tukey-Kramer test (C-F). *P ≤ 0.05 **P ≤ 0.01, ***P ≤ 0.001.