Table 3. Diversity, frequency, and main molecular features of Giardia duodenalis sequences at the gdh, bg, and tpi loci generated in the present study.

GenBank accession numbers are provided. Shushtar County (Iran), 2017–2018.

Locus	Assemblage	Sub-assemblage	No. of isolates	Reference sequence	Stretch	Single nucleotide polymorphisms	GenBank accession number
gdh	A	AII	2	L40510	67–496	C492T	LC504257
			1	L40510	67–496	C85Y, C492T	LC505038
			1	L40510	67–496	T469W, T492Y	LC505039
			1	L40510	67–496	None	LC504259
	B	BIII/BIV	1	L40508	79–491	T186C, C276T, T390C, A441G	LC504260
			1	L40508	79–491	T138C, C231G, C258T, C276T, A462G, T496Y	LC504261
			1	L40508	55–491	C63T, C75T, C123Y, T135Y, T183C, G186R, C255Y, C273Y, C345Y, T366Y, C372Y, T387C, C396Y, A438R	LC504262
bg	A	AII	1	AY072723	63–500	None	LC504266
		AIII	2	AY072724	63–500	None	LC504267
	B	B	1	AY072727	63–500	A183G	LC504265
			1	AY072727	63–526	C123T	LC504263
			1	AY072727	63–526	A119R, C165T, C249T, G289A, C309T, C518T	LC504264
			1	AY072727	105–550	A183G, C256Y, C348T, C564Y	LC505040
tpi	A	AII	2	U57897	288–798	None	LC504269
			2	U57897	304–790	C374M	LC505041
			1	U57897	290–790	C723T	LC504270
			1	U57897	275–805	C287G, G705A	LC504271
			1	U57897	294–807	G726A	LC504272
			1	U57897	300–790	T602Y, C635Y, G746A	LC505051
	B	BIII	1	AF069561	318–795	T294C, G370A	LC504274
			1	AF069561	318–775	G105A	LC504273
			1	AF069561	322–775	C30Y, C104T, C107Y, C137Y, C179Y, C210Y, T377Y	LC505042
			1	AF069561	318–775	A135R, G305R, C336Y	LC505043
			1	AF069561	327–775	T264C	LC504275
			1	AF069561	330–775	T264Y, T294Y	LC505045
			1	AF069561	318–775	C34T, T69Y, G105A	LC505046
			1	AF069561	327–775	C108T	LC504276
		BIV	1	AF069560	297–775	C63M, A174G, A393G	LC505049
		BIII/BIV	1	AF069561	295–775	A41R, C46Y, T57Y, T131Y, T134Y, A162R, C164Y, C171Y, A176G, C298Y, A395G	LC505044
			1	AF069561	295–775	A5R, T57Y, A60R, G128R, T131Y, T134Y, A176G, C194Y, G200R, A395G, G422R	LC505050