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. 2020 Jan 27;14(1):e0008012. doi: 10.1371/journal.pntd.0008012

Fig 1. RpATG8 is highly expressed in the ovaries of vitellogenic females and autophagosomes are formed during vitellogenesis in the oocytes.

Fig 1

A. RpATG8 mRNA quantification in the different organs of vitellogenic females dissected 7 days after the blood meal. B. RpATG8 mRNA quantification in the different components of the ovariole: Tropharium, pre-vitellogenic oocytes, vitellogenic oocytes and chorionated oocytes. The relative expression was quantified using the ΔΔCT method. Graphs show mean ± SEM (n = 6). C. Immunoblotting using the antibodies raised against RpATG8 (LC3). Lanes 1–3: Pre vitellogenic, early vitellogenic and late vitellogenic oocytes. RpATG8-II: RpATG8 conjugated to the phosphatidylethanolamine. D. Immunoblotting densitometry showing the ratio of RpATG8-II/RpATG8-I (n = 3). *p<0.05, **p<0.01. One Way ANOVA.