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. 2020 Jan 27;14(1):e0008012. doi: 10.1371/journal.pntd.0008012

Fig 4. Silencing of RpATG8 does not affect degradation of the main yolk macromolecules during follicular atresia.

Fig 4

A. Total protein quantifications in vitellogenic, control atretic (Type1) and silenced atretic (Type2) oocytes (n = 7). B. 10% SDS-PAGE showing the protein profile of vitellogenic and both types of atretic oocytes (n = 3). Arrows indicate vitellogenin subunits. C. TAG content detected in vitellogenic and both types of atretic oocytes (n = 6). D. PolyP content detected in vitellogenic and both types of atretic oocytes (n = 4). E. The yolk organelles from each of the oocytes (vitellogenic, atretic type-1 and atretic type-2) were incubated with 5μg/ml Acridine Orange (AO) and observed under the fluorescence microscope (n = 5). Bars: 50 μm. *p<0.05, ***p<0.001, One Way ANOVA.