Table 1. Primers sequences.

Primer	Eficiency	Forward	Reverse
RpATG8 (RT-qPCR)	94%	5’-GAACAATGTAATCCCACCGACAAG-3’	5’-CCATAGACATTTTCATCACTATACGC-3’
Rp18S (RT-qPCR)	100.4% [49]	5’-TCGGCCAACAAAAGTACACA-3’	5’-TGTCGGTGTAACTGGCATGT-3’
RpEF1 (RT-qPCR)	93% [49]	5′-GATTCCACTGAACCGCCTTA- 3’	5′-GCCGGGTTATATCCGATTTT-3’
RpAtg8 (dsRNA)	-	5’-TAATACGACTCACTATAGGGTACTATGAA GTTTCAATATAAAGAAGAGC-3’	5’-TAATACGACTCACTATAGGGTACTATCTT CTTCATGATGTTCCTGAT-3’

All sequences were obtained from Vector Base (https://www.vectorbase.org/) and primers were synthesized by Macrogen or IDT technologies. RpATG8 (RP-1485), RpEF1 and Rp18S [49]. The T7 sequence is underlined.