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. 2020 Jan 27;16(1):e1008277. doi: 10.1371/journal.ppat.1008277

Fig 6. The effect of IP6 on other lentiviruses.

Fig 6

(A) Comparison of the MHR sequence and the CASP junction of lentiviruses. Blue bars indicate the location of known (EIAV and HIV-1) and predicted IP6 interacting lysine residues. (B-E) In vitro assembly results of HIV-2, SIV, FIV, and BIV Gag constructs without (red) and with (blue) IP6 at 22°C. (B, C) HIV-2 and SIV assembly was done in 50 mM Tris pH 8, 100 mM NaCl, with GT25 oligo by dialysis. (D) FIV assembly was done in 50 mM Bis-Tris propane, 150 mM NaCl, GT50 oligo by dilution. (E) BIV assembly was done in 50 mM MES pH 6.5, 100 mM NaCl, GT50 oligo by dilution. The mean value of counted particles is given in italics in the bar charts.