Table 1.
Cryo-EM data collection, reconstruction, and modeling statistics
| Data collection | Data Collection 1 | Data Collection 2 | |
|---|---|---|---|
| Microscope | Titan Krios | Titan Krios | |
| Voltage (kV) | 300 | 300 | |
| Magnification | 130,000X | 130,000X | |
| Camera | K2 Summit | K2 Summit | |
| Energy filter slit | 20 eV | 20 eV | |
| Defocus range | 1.2–2.2 (underfocus) | 1.5–2.5 (underfocus) | |
| Pixel size | 1.0605 | 1.0605 | |
| Images (number) | 3260 | 3711 | |
| Reconstruction | CBF3 core | CBF3 Ndc10 D1 | CBF3 Ndc10 D1D2 |
| Particles | 130,143 | 63,177 | 63,177 |
| Box size | 288 | 288 | 288 |
| Accuracy of rotations | 2.56 | 3.32 | 2.27 |
| Accuracy of translations | 0.91 | 1.23 | 1.41 |
| Map resolution—FSC threshold 0.143 | 3.97 | 4.07 | 4.30 |
| Map sharpening B factor | 100 | 80 | 10 |
| Model refinement and statistics | |||
| Refinement resolution | 4.0 | 4.1 | |
| CC (mask) | 0.7985 | 0.78 | |
| R.M.S. deviations | |||
| Bond lengths | 0.011 | 0.007 | |
| Bond angles | 1.199 | 0.942 | |
| Validation | |||
| MolProbity scores | 1.91 | 1.96 | |
| Clashscore | 6.57 | 6.86 | |
| Favored rotamers | 95.34% | 95.81% | |
| Poor rotamers | 0.08% | 0.07% | |
| Cbeta deviations 0.25Å | 0 | 0 | |
| Ramachandran plot | |||
| Favored (%) | 89.99% | 88.63% | |
| Allowed (%) | 9.79% | 11.10% | |
| Disallowed (%) | 0.22% | 0.27% | |
| Peptide omega | |||
| Cis prolines (%) | 0.00% | 0.00% | |
| Twisted peptides | 0.15% | 0.20% |
FSC, Fourier shell correlation