Figure 5.
KO of Skn-1a does not affect responsiveness of geniculate ganglion neurons to ATP or 5-HT. Skn-1a-/- mice were crossed to 5-HT3A-GFP mice. A, B, Raw calcium imaging traces of isolated, individual GFP+ (green) or GFP- (black) geniculate ganglion neurons in response to 10 μM ATP, 10 μM 5-HT, or 55 mM KCl. C, D, Summary of responses to exogenous stimuli; N = 7 GFP+, 4 GFP- from 3 Skn-1a-/-/5-HT3A-GFP, and 6 GFP+ and 6 GFP- from 3 Skn-1aWT/5-HT3A-GFP. Bar charts indicate sample mean, error bars represent SEM, and superimposed dot plot represents each individual cell; *p <0.05 by Two-way ANOVA with Holm–Sidak post hoc test.