Figure 7.
5-HT3A-GFP innervation in Skn-1a-/- mice. 5-HT3A-GFP mice were crossed with Skn-1a-/-. Skn-1a-/- mice that had the GFP transgene and WT littermates that also had the GFP transgene were used for experiments. A, B, Maximal z-projections of fungiform taste buds from WT and KO mice labeled with antibodies against GFP (green) and P2X3 (magenta). A’, B’, Same image as A, B but after prepressing which included Otsu thresholding. C, Analysis was performed on each optical plan of individual taste buds to quantify the relative immunoreactivity of P2X3 and GFP; *p < 0.05 by Kruskal–Wallis test followed by Dunn test for multiple comparisons. Three to four mice from each genotype were used, and 228 taste buds were quantified.