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. Author manuscript; available in PMC: 2020 Feb 6.
Published in final edited form as: Cell Rep. 2020 Jan 7;30(1):69–80.e6. doi: 10.1016/j.celrep.2019.12.003

Figure 4. Reducing c-Myc Expression Largely Rescues Serial Reconstituting Activity of Aarssti/sti HSCs.

Figure 4.

(A) Western blot examining c-Myc in 104 HSCs/MPPs isolated from wild-type (+/+), Mx1-Cre+;Aarssti/sti;Mycfl/+ (sti/sti;Myc+/−) and Aarssti/sti (sti/sti;Myc+/+) mice.

(B) Diagram showing bone marrow transplantation strategy.

(C) Donor cell engraftment 16 weeks after 5 × 105 bone marrow cells from wild-type (+/+), Aarssti/sti (sti/sti;Myc+/+) and Mx1-Cre+;Aarssti/sti;Mycfl/+ (sti/sti;Myc+/−) mice were transplanted with 5 × 105 recipient-type bone marrow cells into irradiated mice. Total hematopoietic cells, B cell, T cell, and myeloid cell engraftment is shown (n = 4–8 donors and 26–44 total recipients/genotype).

(D) Donor cell engraftment in secondary recipients (n = 4–10 donors and 17–44 recipients/genotype).

(E) Frequency of secondary recipients in (D) that exhibited long-term multilineage reconstitution.

Data represent mean ± standard error of the mean. Statistical significance was assessed using a Student’s t test (C and D) or Fisher’s exact test (E). *p < 0.05; **p < 0.01; ***p < 0.001.