Figure 5.

miR‐30e‐5p suppresses angiogenesis in squamous cell carcinoma of head and neck (SCCHN) in vivo. A, Matrigel angiogenesis plug assay was formed by subcutaneously implanting Fadu cells with Matrigel. B and C, Gel plugs were collected and photographed (B) in 7 d after implantation; the proangiogenic factors were detected by quantitative PCR detection (C). D‐J, H&E staining and immunohistochemical staining analysis of the levels of CD31 and vascular endothelial growth factor (VEGF) in gel plugs (D) and xenograft tumors (E) of nude mice. Arrows are pointed to neovascularization and quantification of the microvessel density (F, G, I, J). The positive staining cell numbers of CD31 were counted (H). K and L, chick chorioallantoic membrane (CAM) angiogenesis assays were performed with Fadu cells stably overexpressing miR‐30e‐5p or vector. Representative images of new blood vessel formation (K) and quantification of the average number of new blood vessels (L; n = 10 for each group). *P < 0.05; **P < 0.01