Figure 1.

Protein expression of UCHL1, p53 and RB1, and mRNA expression of UCHL1 in various lung cancer cell lines and normal lung epithelial cells. A, Increased UCHL1 levels in all small cell lung cancers (SCLC) tested (H82, H69 and H526) and in three non–small cell lung cancer (NSCLC) cell lines (A549, H1299 and PC9) was confirmed by western blotting. B, Comparison of UCHL1/β‐actin protein expression ratio among the cell lines showed that SCLC consistently had high UCHL1 levels, whereas NSCLC showed different expression patterns depending on the cell line. C, Comparison of UCHL1 mRNA expression among the cell lines showed that all lung cancer cell lines except for HCC827 had high UCHL1 levels