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. 2020 Jan 13;111(2):451–466. doi: 10.1111/cas.14279

Figure 2.

Figure 2

PLK2, PTP4A1 and RPS27L are Δ1stTAD‐p53‐inducible genes. A‐C, Expression of PLK2 (A), PTP4A1 (B) and RPS27L (C) was analyzed by microarray expression analysis. Temperature‐sensitive wild‐type‐expressing, Δ1stTAD‐p53‐expressing or control Saos2 cells were tested for induction of PLK2, PTP4A1 and RPS27L upon temperature shift to the permissive temperature with or without γ‐ray irradiation. Cells were subjected to γ‐ray irradiation (30 or 10 Gy) 2 h after temperature shift to 32°C. Cells were collected 5 or 6.5 h post–temperature shift. Relative induction of the genes in three cell lines upon temperature shift is shown. D‐G, H1299 cells were infected with adenoviruses expressing control Lac Z or Δ1stTAD‐p53 at MOI (multiplicity of infection) 6, and harvested 48 h post–infection. Expression of Δ1stTAD‐p53 was analyzed by western blotting (D). PLK2 (E), PTP4A1 (F) and RPS27L (G) mRNA levels were analyzed by quantitative RT‐PCR.