Figure 4.
Transcription of PLK2, PTP4A1 and RPS27L are induced in a manner dependent on the activity of the 2nd transactivation domain of p53. A, Schema of p53 constructs used in this study. B, G, Expression of p53 constructs was analyzed by western blotting. (C‐F, H‐K), H1299 cells were transfected with the indicated p53 constructs and harvested 24 h post–transfection. PLK2 (C, H), PTP4A1 (D, I), RPS27L (E, J) and PHLDA3 (F, K) mRNA levels were analyzed by quantitative RT‐PCR