Figure 1.

Classification of 88 NSCLC cell lines according to expression levels of representative NRF2 target genes. A, Comparison of cell rankings according to expression levels of 15 and 4 representative NRF2 target genes. The continuous red to blue color code represents the ranking, in which the higher ranking indicates the higher expression levels of NRF2 target genes. B, Classification of the cells according to the 4 representative NRF2 target genes, NQO1, GCLC, GCLM and SLC7A11. Cells indicated in pink, grey and light green are NRF2‐high, NRF2‐middle and NRF2‐low cell lines, respectively. Red highlight indicates cells that belong to NRF2‐high cells but do not possess non–synonymous mutations in the KEAP1, NRF2 or CUL3 genes. The continuous red to blue color codes represent a cell ranking according to the expression level of each NRF2 target gene, which are available in the CCLE database. Non–synonymous mutations in the KEAP1, NRF2 and CUL3 genes, which were identified in our exome analysis, are indicated. A CUL3 mutation, 567V>I, is indicated with a faint yellow color because judging from the high frequency, this mutation is likely to be a genetic polymorphism. The rightmost column indicates the histology type of a tumor, from which each cell line was derived. ADC, adenocarcinoma; ADSC, adenosquamous cell carcinoma; LCC, large cell carcinoma; NSCLC, non–small‐cell carcinoma without detailed information; SCC, squamous cell carcinoma