Figure 2.

Distribution of cancer stem‐like cells (CSC) in the layers of the spheroids. A, In the spheroids with mixed CSC and NSCC at 1:1, the red CSCs gradually diffused to the outer layer of the spheroids during the invasion process. B and D, The yellow circles in the 0 h and 72 h figures were several circles with radius of which increased by 10 μm in turn from the center. The image is representative of 72 samples. C and E, The area of pixels occupied by cell distribution in enlarged ring at 0 h and 72 h. n = 30