Table 2. Selected attributes of Anisakis spp. seropositive individuals working in Croatian fish processing industry.
| serum # | birth year | gender | center & facility | job title | free-time fishing | raw/salted/marinated fish consumption | frequency of fish consumption | preparation of dish | type of consumed fish | acute allergy symptoms | chronic diseses | Ani s 1 | Ani s 7 | ImmunoCAP |
Trichinella ELISA |
Trichinella WB |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 52 | 1959 | M | 1A | fish seller | + | + | few x/ week | B, G, M | SPF, FF | U, Rh | CV | 1.888 | 0.934 | 9.75 | - (4.9%) | - |
| 111 | 1966 | F | 1A | fish cleaning and salting | - | + | 1x/ week | B, G, R | SPF | U | RA | 0.006 | 0.124 | 0.54 | - | - |
| 128 | 1969 | F | 1A | fish sorting | - | + | every day | B | SPF, FF | C | CA | 0.135 | 0.352 | 17.0 | + (14.3%) | - |
| 216 | 1959 | M | 2C | owner | + | + | 1x/ week | G, M | FF | C, Rh | - | 0.004 | 0.503 | 4.99 | + (18.6%) | - |
| 244 | 1979 | M | 1A | fish processing | + | + | 1x/ week | G | SPF | - | CV | -0.003 | 0.161 | 1.94 | + (16.6%) | - |
| 310a | 1954 | M | 3E | can sterilisation | + | + | few x/ week | B, G | SPF | - | - | 0.004 (0) |
0.06 (0.042) |
0.24 | + (23.9%) | - |
| 321 | 1971 | F | 3E | packaging | + | + | few x/ week | B | SPF | - | P | 0.873 | 1.212 | 3.66 | - | - |
| 446 | 1957 | M | 3H | load of raw fish | + | - | 1x/ week | G | FW | C, Rh, DC | CA | -0.003 | 0.105 | 0.23 | - | - |
| 453 | 1957 | M | 3H | unloading of raw fish | - | - | 1x/ week | B, G | SPF | U | - | -0.006 | 0.198 | 0.89 | - | - |
| 525 | 1961 | F | 4J | commerce | - | - | 1x/ week | B, G | SPF, FF | - | - | -0.002 | 0.213 | <0.1 | - | - |
| 595 | 1987 | F | 4J | fish sorting | - | - | few x/ week | G | SPF | U | D, T | 0.132 | 0.173 | <0.1 | + (17.8%) | - |
Center 1 Brac: A Sardina facility; Center 2 Sinj: B Conex, C Felicita, D Trenton; Center 3 Zadar: E Sali Mardesic, F Omega Benkovac, G Mislov, H Ostrea, I Noclerius; Center 4 Rovinj: J Mirna; F: female; M: male; B: boiled fish; G: grilled fish; M: marinated fish; R: raw fish; SPF: small pelagic fish; FF: finfish; FW: freshwater fish; U: urticaria; Ri: rhinitis; C: conjunctivitis; DC: dry cough; CV: cardiovascular diseases; CA: chronic allergy; P: psychosis; D: diabetes, T: thyroidal imbalance; RA: rheumatoid arthritis. The values in Ani s 1 and Ani s 7 columns show ODs obtained in ELISA for each serum (positives in bold). Cut-off values were OD = 0.09 for Ani s 1 and OD = 0.05 for Ani s 7. The values in ImmunoCAP column show antibodies kU/L obtained using the whole-nematode extract by commercial ImmunoCAP for each serum (positives in bold). Cut-off value is 0.35 kU/L. Cut-off values (shown in parenthesis) for Trichinella ELISA was 11.8%. In bold: seropositive. aborderline serum. In parenthesis are Ani s 1 and Ani s 7 OD values of the second testing.