Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Genetic reagent (Drosophila melanogaster) | pkpk-sple13 | Gubb et al., 1999, PMID: 10485852 | BDSC:41790; FLYB:FBal0060943; RRID:BDSC_41790 |
FlyBase symbol: pkpk-sple-13 |
| Genetic reagent (Drosophila melanogaster) | pkpk-sple14 | Gubb et al., 1999, PMID: 10485852 | FLYB:FBal0035401 | FlyBase symbol: pkpk-sple-14 |
| Genetic reagent (Drosophila melanogaster) | pkpk30 | Gubb et al., 1999, PMID: 10485852 | BDSC:44229; FLYB:FBal0101223; RRID:BDSC_44229 |
FlyBase symbol: pk30 |
| Genetic reagent (Drosophila melanogaster) | pksple1 | Gubb et al., 1999, PMID: 10485852 | BDSC:422; FLYB:FBal0016024; RRID:BDSC_422 |
FlyBase symbol: pksple-1 |
| Genetic reagent (Drosophila melanogaster) | vangA3 | Taylor et al., 1998, PMID: 9725839 | FLYB:FBal0093183 | FlyBase symbol: VangA3 |
| Genetic reagent (Drosophila melanogaster) | vangstbm6 | Wolff and Rubin, 1998, PMID: 9463361 | BDSC:6918; FLYB:FBal0062424; RRID:BDSC_6918 |
FlyBase symbol: Vangstbm-6 |
| Genetic reagent (Drosophila melanogaster) | fzR52 | Krasnow and Adler, 1994, PMID: 7924994 | FLYB:FBal0004939 | FlyBase symbol: fz23 |
| Genetic reagent (Drosophila melanogaster) | dsh1 | Bloomington Drosophila Stock Center | BDSC:5298; FLYB:FBal0003138; RRID:BDSC_5298 |
FlyBase symbol: dsh1 |
| Genetic reagent (Drosophila melanogaster) | UAS-pksple | Bloomington Drosophila Stock Center | BDSC:41780; FLYB:FBti0148928; RRID:BDSC_41780 |
FlyBase symbol: P{UAS-sple+}3 |
| Genetic reagent (Drosophila melanogaster) | UAS-pkRNAi | Vienna Drosophila Resource Center | VDRC:v101480; FLYB:FBst0473353; RRID:FlyBase_FBst0473353 | FlyBase symbol: P{KK109294}VIE-260B |
| Genetic reagent (Drosophila melanogaster) | UAS-fmiRNAi | Bloomington Drosophila Stock Center | BDSC:26022; FLYB:FBti0114752; RRID:BDSC_26022 |
Flybase symbol: P{TRiP.JF02047}attP2 |
| Genetic reagent (Drosophila melanogaster) | UAS-fzRNAi | Bloomington Drosophila Stock Center | BDSC:34321; FLYB:FBti0140932; RRID:BDSC_34321 |
Flybase symbol: P{TRiP.HMS01308}attP2 |
| Genetic reagent (Drosophila melanogaster) | UAS-vangRNAi | Bloomington Drosophila Stock Center | BDSC:34354; FLYB:FBti0140967; RRID:BDSC_34354 |
Flybase symbol: P{TRiP.HMS01343}attP2 |
| Genetic reagent (Drosophila melanogaster) | UAS-dsRNAi | Bloomington Drosophila Stock Center | BDSC:32964; FLYB:FBti0140473; RRID:BDSC_32964 |
Flybase symbol: P{TRiP.HMS00759}attP2 |
| Genetic reagent (Drosophila melanogaster) | UAS-ds | Matakatsu and Blair, 2004, PMID: 15240556 | FLYB:FBtp0019964 | Flybase symbol: P{UAS-ds.T} |
| Genetic reagent (Drosophila melanogaster) | dll-GAL4 | Bloomington Drosophila Stock Center | BDSC:3038; FLYB:FBti0002783; RRID:BDSC_3038 |
Flybase symbol: P{GawB}Dllmd23 |
| Genetic reagent (Drosophila melanogaster) | MS1096-GAL4 | Bloomington Drosophila Stock Center | BDSC:8860; FLYB:FBti0002374; RRID:BDSC_8860 |
Flybase symbol: P{GawB}BxMS1096 |
| Genetic reagent (Drosophila melanogaster) | armP-fz::EGFP | Strutt, 2001, PMID:11239465 | FLYB:FBtp0014592 | Flybase symbol: P{arm-fz.GFP} |
| Genetic reagent (Drosophila melanogaster) | actP-vang::EYFP | Strutt, 2002, PMID: 12137731 | FLYB:FBtp0015854 | Flybase symbol: P{Act5C(-FRT)stbm-EYFP} |
| Genetic reagent (Drosophila melanogaster) | actP > CD2>vang::EYFP | Strutt, 2002, PMID: 12137731 | FLYB:FBtp0084387 | Flybase symbol: P{Act5C(FRT.polyA)stbm-EYFP} |
| Genetic reagent (Drosophila melanogaster) | ci-GAL4 | Croker et al., 2006, PMID: 16413529 | FLYB:FBtp0057188 | Flybase symbol: P{ci-GAL4.U} |
| Genetic reagent (Drosophila melanogaster) | UAS-mCherry | Bloomington Drosophila Stock Center | BDSC:38424; FLYB:FBti0147460; RRID:BDSC_38424 |
Flybase symbol: P{UAS-mCherry.NLS}3 |
| Genetic reagent (Drosophila melanogaster) | actP > CD2>Gal4 | Bloomington Drosophila Stock Center | BDSC:30558; FLYB:FBti0012408; RRID:BDSC_30558 |
Flybase symbol: P{GAL4-Act5C(FRT.CD2).P}S |
| Genetic reagent (Drosophila melanogaster) | UAS-RFP | Bloomington Drosophila Stock Center | BDSC:30558; FLYB:FBti0129814; RRID:BDSC_30558 |
Flybase symbol: P{UAS-RFP.W}3 |
| Antibody | goat polyclonal anti-Su(H) | Santa Cruz | Santa Cruz:sc-15183 RRID:AB_672840 |
1/200 (immunolabelling) |
| Antibody | Mouse monoclonal anti-V5 | Thermo-Fisher | Thermo_Fisher:R960-25, RRID:AB_2556564 | 1/200 (immunolabelling) 1/1000 (Western blotting) |
| Antibody | Guinea pig polyclonal anti-Pk[C] |
Olofsson et al., 2014, PMID: 25005476 |
N/A | 1/800 (immunolabelling) 1/1000 (Western blotting) |
| Antibody | Rat monoclonal anti-dEcad | DSHB | RRID:AB_528120 | 1/200 (immunolabelling) |
| Antibody | Mouse monoclonal anti-γ-Tubulin | Sigma-Aldrich | Sigma-Aldrich: T6557 RRID:AB_477584 |
1/1000 (Western blotting) |
| Recombinant DNA reagent | pCFD4 | Addgene | RRID:Addgene_49411 | CRISPR gRNA backbone |
| Recombinant DNA reagent | pDsRedattp | Addgene | RRID:Addgene_51019 | Donor recombinant DNA backbone |
| Recombinant DNA reagent | pCR-Blunt-II-TOPO | Thermo-Fisher | RRID:Addgene_29705 | Backbone for sub-cloning |
| Sequence-based reagent | pkpk gRNA 1 | This paper | gRNA sequence in PCR primers | ATGGCTCAGGCCCGATCTAG |
| Sequence-based reagent | pkpk gRNA 2 | This paper | gRNA sequence in PCR primers | GTGGATCAACCCCTGGAAAC |
| Sequence-based reagent | pksple gRNA 1 | This paper | gRNA sequence in PCR primers | CTCGTAAATTTAGCTTCGAG |
| Sequence-based reagent | pksple gRNA 2 | This paper | gRNA sequence in PCR primers | AGATGCAATTTGGCCGCCCT |