Figure 1. Cytoprotective effects of BCE treatment on H2O2-stimulated L6 cells. L6 cells (1 × 105) were treated with hydrogen peroxide (H2O2, 300 μM), with or without Black chokeberry ethanol extract (BCE: 100, 300, and 1000 μg/mL) for 24 h. (A) The cells were incubated with 2,3-bis-(2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5-carboxanilideat 37°C for 4 h. Absorbance was measured at 450 nm using a plate reader. Bar graphs indicate the percentage of cell viability. Data are expressed as the mean ± SE values from three independent experiments. *P < 0.05 compared to only H2O2 treated group. (B) The Live/Dead cell reagents were dispensed into each well, and these images were acquired using a fluorescence microscope (excitation 580 nm and emission 604 nm).
