FIGURE 2.

CJ exacerbates liver damage and aggravates animal survival in small partial liver graft. A, The 7-day survival rates in the 30% CC and 30% CJ groups were 100% (10/10) and 50% (5/10), respectively, with a significant difference (P = 0.011, log-rank test). B, Representative HE staining of the graft liver in the 30% CJ and 30% CC groups at 24 and 72 h post-LT. The 30% CJ group showed severe liver damage, as confirmed by moderate vacuolization and moderate necrosis at 24 h post-LT and moderate vacuolization and moderate necrosis at 72 h post-LT. The 30% CC group showed mild vacuolization and no necrosis at 24 h post-LT and minimal vacuolization and no necrosis at 72 h post-LT. The original magnification was ×100 for both images. The scale bar in each panel represents 100 μm. C and D, Highly elevated serum AST and ALT levels at 12 h post-LT were immediately improved at 24 h post-LT in the 30% CC group while elevated AST and ALT levels persisted until 72 h post-LT in the 30% CJ group. Serum AST and ALT levels at 72 h post-LT were significantly higher in the 30% CJ group than in the 30% CC group. *P < 0.05, †P < 0.01, Mann-Whitney U test. n = 5/group. E, Serum T-bil level was elevated only in the 30% CJ group. Serum T-bil level at 72 h post-LT was significantly higher in the 30% CJ group than in the 30% CC group. *P < 0.05, Mann-Whitney U test. n = 5/group. F, Bile production volume was significantly less in the 30% CJ group than in the 30% CC group at 24 and 72 h post-LT (24 h: P = 0.008, 72 h: P = 0.008). *P < 0.05, †P < 0.01, Mann-Whitney U test. n = 5/group. 30% CC, arterialized 30% partial liver transplantation with choledocho-choledochostomy; 30% CJ, arterialized 30% partial liver transplantation with choledocho-jejunostomy; ALT, alanine aminotransferase; AST, aspartate aminotransferase; CJ, choledocho-jejunostomy; HE, hematoxylin-eosin; LT, liver transplantation; T-bil, total bilirubin.