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. 2019 Jul 23;69(1):26–33. doi: 10.1538/expanim.19-0005

Fig. 1.

Fig. 1.

PCR products from genomic DNA of Ccdc85c(A). Ccdc85c KO rats have an approximately 350-bp deletion in Ccdc85c. Immunofluorescence staining for CCDC85C protein in the duodenum (B–D). CCDC85C is expressed in the epithelial cells in wild type (WT) (C) and heterozygous (D) rats at 17W. CCDC85C expression was not observed in Ccdc85c KO rats at four weeks of age (4W) (B). Scale bar, 25 µm. Sequences of the WT and Ccdc85c KO rat PCR products (E). A 355-bp genomic sequence encompassing exon 1 is deleted in Ccdc85c KO rats (g. 132,183,075-132,183,429del355; NCBI NC_005105.4).