Skip to main content
. Author manuscript; available in PMC: 2021 Feb 1.
Published in final edited form as: Hypertension. 2019 Dec 23;75(2):492–499. doi: 10.1161/HYPERTENSIONAHA.119.14062

Figure 4.

Figure 4.

CLGN overexpression increased aldosterone production in HAC15 cells. (A, B) After confluent HAC15 cells with control or CLGN were serum deprived for 24 h, cells were incubated with fresh media with 0.1% serum for 24 h. Relative aldosterone and cortisol levels in the media are compared between HAC15 cells with control and CLGN. *P < 0.05 vs. control (n = 3). (C) Confluent HAC15 cells with control or CLGN were serum deprived for 24 h, and the serum-deprived cells were harvested for CYP11B2 and GAPDH mRNA levels were measured. *P < 0.05 vs. each control (n = 3). (D) After transduction of control or CLGN in HAC15 cells, R-CEPIAer was applied to compare intra-endoplasmic reticulum Ca2+ levels between control and CLGN cells (n = 3).