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. Author manuscript; available in PMC: 2021 Feb 1.
Published in final edited form as: Hypertension. 2019 Dec 23;75(2):492–499. doi: 10.1161/HYPERTENSIONAHA.119.14062

Figure 5.

Figure 5.

Steroidogenic enzymes and StAR protein expression in HAC15 cells with control or CLGN. After confluent HAC15 cells with control or CLGN were serum deprived for 24 h, the cells were harvested for SDS-PAGE and immunoblotting analysis. (A) The band intensities of CYP11B2, HSD3B2, StAR and β-actin were depicted. (B-D) CYP11B2, HSD3B2 and StAR protein levels normalized by β-actin levels were compared in between control and CLGN over-expressing cells. *P < 0.05 vs. control (n = 3). (E) Protein aggregation measured using the Proteostat Protein Aggregation Assay Kit (Enzo Life Sciences, Farmingdale, NY, USA) following the manufacturer's instructions (n = 3).