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. 2019 Dec 25;118(3):708–719. doi: 10.1016/j.bpj.2019.12.017

Figure 2.

Figure 2

Example of variations in ice thickness over a grid. (A) Cryo-EM “grid atlas” micrograph of an aqueous film prepared using blotting shows regions of high and low electron transmission. Scale bars, 400 μm. (B) A region of interest from the grid atlas is shown at a higher magnification. Scale bars, 100 μm. (C) Cryo-SEM image of the same specimen, with several regions of thick ice, corresponding to local regions of low electron transmission. Scale bars, 400 μm. (D) The same region of interest from the cryo-SEM image is shown. Scale bars, 100 μm. To see this figure in color, go online.