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. Author manuscript; available in PMC: 2021 Feb 4.
Published in final edited form as: Cell Metab. 2019 Dec 5;31(2):339–350.e4. doi: 10.1016/j.cmet.2019.11.012

Figure 1: Keap1 loss increases dependency on exogenous supply of NEAAs.

Figure 1:

a) In vitro uptake assay of amino acids after 24 hours b) Serum levels of asparagine, glycine and serine in mice bearing subcutaneous tumors. c) Proliferation in media lacking specified amino acid. d) Relative viability of cells cultured treated with L-asparaginase for 3 days. e) Proliferation of wildtype (Wt) or Keap1 mutant (Mut) LKR (KrasG12D/+;p53+/+) cell lines in media lacking serine or asparagine. f) Proliferation of cells expressing an empty vector or WT Keap1 in RPMI lacking serine or asparagine. g) Schematic depicting synthesis of serine from glucose (top) and asparagine from glutamine (bottom). Filled blue circles represent 13C atoms derived from [U13C]-D-glucose or [U13C]-L-glutamine. h) Mass isotopomer analysis of serine and asparagine in cells cultured in complete or amino acid deprived conditions. *p<0.05, **p<0.01, ***p<0.001, ****p<0.0001.