Table 1.

Distribution of the number of probes on the Axiom^® Wheat‐Relative Genotyping Array with BLASTN hit to a single contig in the wheat genome, the primers designed from these probes, PCR products sent for sequencing, the SNPs discovered and selected for KASP assay design

Wheat chromosome	Probes on WRGA† chip with blast to 1 contig‡	Primer pairs designed	Primers worked	PCR products sent for sequencing	Successfully sequenced samples	Successfully sequenced samples (%)	SNPs discovered (across all 10 species)	SNPs sent for KASP assay design
1A	83	63	54	416	276	66.35	93	45
1B	141	99	78	507	312	61.54	135	56
1D	144	99	83	608	334	54.93	154	46
2A	112	91	79	540	396	73.33	119	48
2B	159	132	99	605	442	73.06	141	54
2D	130	111	93	633	447	70.62	126	43
3A	85	86	86	747	278	37.22	117	45
3B	294	266	192	1469	1009	68.69	168	53
3D	79	76	62	469	296	63.11	126	45
4A	95	77	75	833	447	53.66	96	46
4B	88	73	57	415	224	53.98	65	35
4D	73	64	67	586	329	56.14	90	43
5A	68	55	46	382	262	68.59	78	49
5B	167	122	112	833	530	63.63	115	56
5D	155	110	97	729	426	58.44	116	47
6A	102	97	83	642	501	78.04	113	45
6B	92	84	72	531	348	65.54	107	53
6D	140	128	119	897	604	67.34	125	49
7A	90	60	49	403	220	54.59	73	44
7B	140	89	74	521	301	57.77	111	47
7D	279	188	44	965	469	48.60	106	51
Total	2716	2170	1721	13 731	8451	61.67	2374	1000

^†Axiom^® Wild Relative Genotyping Array.

^‡BLASTN against IWGSC CSS v3.